厉文成;张庆华;王柯;毛忠贵;唐蕾;张建华;张宏建;

• 1:江南大学工业生物技术教育部重点实验室
• 2:江南大学生物工程学院
• 3:江西农业大学生物科学与工程学院

摘要(Abstract)：

传统培养木薯渣降解复合菌系的蛋白胨纤维素(PCS)培养基成本较高,其主要成本来源于氮源——蛋白胨和酵母粉,限制了复合菌系的规模化应用。为解决此问题,从对木薯渣降解复合菌系RXS生长、产酶及底物降解的影响3个方面比较了胰蛋白胨/酵母粉及8种廉价氮源。结果表明,比较适宜的廉价氮源为鱼粉和玉米浆。接着探究了二者的复配比例对RXS的影响,并获得一种廉价培养基。考虑到为调节p H值和促进产酶添加的试剂对甲烷菌的毒害作用,以高温厌氧消化出水作为配料水以调节p H值,并比较了RXS在廉价培养基和PCS培养基上对木薯渣的降解效果。最终所得廉价培养基组成为木薯渣10 g/L、滤纸5 g/L、鱼粉4 g/L、玉米浆3.5 g/L,高温厌氧消化出水作为配料水。经成本核算,廉价培养基的成本仅为PCS培养基的1/6。并且复合菌系在廉价培养基上对木薯渣的降解率较PCS培养基提高了约37%。就工业化生产而言,该培养基不仅显示了对木质纤维素物质较好的降解效果,还节约了成本和减少了水的用量。

关键词(KeyWords)： 环境工程学;复合菌系;氮源;木薯渣;底物失重率

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(31260024);; 江苏省科技支撑计划项目(BE2011623);; 江苏省环保科研课题(2012047)

作者(Authors): 厉文成;张庆华;王柯;毛忠贵;唐蕾;张建华;张宏建;

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