郭浩;夏慧丽;王书琪;赵晓祥;

• 1:东华大学环境科学与工程学院
• 2:台州市质量技术监督检测研究院

摘要(Abstract)：

从污水处理厂活性污泥中筛选得到一株多溴联苯醚好氧降解菌,命名为GH10。根据菌株形态特征、生理生化特性、16S r DNA基因序列及系统发育树分析,鉴定为长野雷夫松氏菌(Leifsonia shinshuensis)。该菌能够利用十溴联苯醚作为唯一碳源生长,它对十溴联苯醚的降解率用HPLC法测定。十溴联苯醚质量浓度为10⁵0 mg/L时,降解效果较好,在十溴联苯醚初始质量浓度为50 mg/L、温度为30℃、摇床转速为150 r/min的条件下避光培养5 d,菌株GH10对十溴联苯醚的降解率可达到59.24%。降解十溴联苯醚的最适条件为温度30℃,p H值7.0,接种量10%,相同条件下添加外加碳源葡萄糖可提高其降解率到90.08%。通过对十溴联苯醚降解动力学的模拟发现,添加外加碳源葡萄糖可以将十溴联苯醚的半衰期从3.91 d缩短至1.45 d。

关键词(KeyWords)： 环境工程学;微生物;多溴联苯醚;生物降解;降解特性

Abstract:

Keywords:

基金项目(Foundation): 上海市基础研究重点项目(09JC1400600)

作者(Authors): 郭浩;夏慧丽;王书琪;赵晓祥;

参考文献(References)：

• [1]HARRAD S,DIAMOND M.New directions:exposure to polybrominated diphenyl ethers(PBDEs)and polychlorinated biphenyls(PCBs):current and future scenarios[J].Atmospheric Environment,2006,40(6):1187-1188.
• [2]LIU Hanxia(刘汉霞),ZHANG Qinghua(张庆华),JIANG Guibin(江桂斌),et al.Polybrominated diphenyl ethers and its related environmental problems[J].Progress in Chemistry(化学进展),2005,17(3):554-561.
• [3]REN Jinliang(任金亮),WANG Ping(王平).Progress and characteristics of environmental behavior of polybrominated diphenyl ethers[J].Chemical Industry and Engineering Progress(化工进展),2006,25(10):152-158.
• [4]GOUIN T,HAMER T.Modeling the environmental fate of the polybmminated diphenyle ethers[J].Environmental International,2003,29(6):717-724.
• [5]DE WIT C.An overview of brominated flame retardant in the environment[J].Chemosphere,2002,46(5):583-624.
• [6]MA Jin,QIU Xinghua,ZHANG Jinliang,et al.State of polybrominated diphenyl ethers in China:an overview[J].Chemosphere,2012,88(7):769-778.
• [7]BI X,THOMAS G O,JONES K C,et a1.Exposure of electronics dismantling workers to polybrominated diphenyl ethers,polychlorinated biphenyls,and organochlorine pesticides in South China[J].Environmental Science&Technology,2007,41(16):5647-5653.
• [8]KEUM Y S,LI Q X.Reduetive debromination of polybrominated diphenyl ethers by zerovalent iron[J].Environmental Science&Technology,2005,39(7):2280-2286.
• [9]SHIH Y-H,WANG C-K.Photolytic degradation of polybromodiphenyl ethers under UV-lamp and solar irradiations[J].Journal of Hazardous Materials,2009,165(1/2/3):34-38.
• [10]SUN Yunna(孙云娜),WEI Dongyang(魏东洋),LI Jie(李杰),et a1.The research of polybrominated diphenyl ethers(PBDEs)degradation[J].Guangdong Chemical Industry(广东化工),2011,38(5):5-6.
• [11]KIM Y-M,MURUGESAN K,CHANG Y-Y,et al.Degradation of polybrominated diphenyl ethers by a sequential treatment with nanoscale zero valent iron and aerobic biodegradation[J].Journal of Chemical Technology and Biotechnology,2012,87:216-224.
• [12]SHIH Y-H,CHOU H-L,PENG Y-H,et al.Synergistic effect of microscale zerovalent iron particles combined with anaerobic sludges on the degradation of decabromodiphenyl ether[J].Bioresource Technology,2012,108:14-20.
• [13]KIM Y M,NAM I H,MURUGESAN K,et al.Biodegradation of diphenyl ether and transformation of selected brominated congeners by Sphingomonas sp.PH-07[J].Applied Microbiology and Biotechnology,2007,77(1):187-194.
• [14]DONG Xiuzhu(东秀珠),CAI Miaoying(蔡妙英).Identification manual of common bacteria system(常见细菌系统鉴定手册)[M].Beijing:Science Press,2001:258-260.
• [15]ZHANG Xiangrong(张向荣),XUE Keshe(薛科社).Determination of polybrominated diphenyl ethers(PBDEs)in sediment by HPLC[J].Chinese Journal of Spectroscopy Laboratory(光谱实验室),2009,26(4):986-989.
• [16]DING J,ZHOU J,JIANG W Y,et al.Aerobicmicrobial degradation of polybrominated diphenyl ethers[J].Chinese Journal of Environmental Science,2008,29(11):3179-3184.
• [17]JIANG Hong(江虹),YIN Hua(尹华),PENG Hui(彭辉),et al.Way to isolate a BDE209 aerobic bio-degrading strain and its degradation properties[J].Journal of Safety and Environment(安全与环境学报),2010,10(2):49-53.
• [18]YANG Liuyan(杨柳燕),MA Wenyi(马文漪).Environmental microbiology engineering(环境微生物工程)[M].Nanjing:Nanjing University Press,1996:40-42.
• [19]ZANG Shuyan(臧淑艳),LI Peijun(李培军),ZHOU Qixing(周启星),et al.Study on continued degradation of Ba P and its metabolites[J].Environmental Science(环境科学),2006,27(12):2531-2535.