p,p'-DDT对大肠癌细胞DLD1细胞黏附的影响Effect of p,p'-DDT on the cell adhesion of the colon cancer cell DLD1
赵君宇,刘建新,晋小婷,贾慧,贾丹,李丹丹,李卓玉,宋莉
摘要(Abstract)：
为了探讨p,p'-DDT对大肠癌细胞黏附的影响及机制,以肠癌细胞DLD1为试验材料,采用细胞聚集试验、细胞与基质间黏附分析方法,研究了1 nmol/L的p,p'-DDT作用于DLD1细胞48 h后,p,p'-DDT对DLD1细胞-细胞黏附和细胞-基质黏附的影响。通过Real time PCR和Western blot方法检测了细胞黏附关键因子E-cadherin、CD29的表达变化。结果表明:1 nmol/L的p,p'-DDT作用于DLD1细胞48 h后,DLD1细胞-细胞黏附率明显降低(p<0.01),细胞-基质黏附率明显升高(p<0.01);1 nmol/L的p,p'-DDT作用于DLD1细胞48 h后,降低了E-cadherin的表达水平,同时提高了CD29的表达水平。研究表明,p,p'-DDT具有降低DLD1细胞-细胞黏附并提高其细胞-基质黏附的作用。p,p'-DDT可能通过改变黏附关键因子E-cadherin和CD29的表达来影响细胞黏附,进而促进肿瘤细胞的浸润和侵袭。
关键词(KeyWords)： 环境学;滴滴涕;DLD1细胞;细胞-细胞黏附;细胞-基质黏附
基金项目(Foundation): 国家自然科学基金项目(31271516,21207084);; 教育部博士点博导基金项目(20111401110011);; 中国博士后基金项目(2012M521178)
作者(Author): 赵君宇,刘建新,晋小婷,贾慧,贾丹,李丹丹,李卓玉,宋莉
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