沈洪艳;曹志会;赵月;王冰;

• 1:河北科技大学环境科学与工程学院
• 2:河北省药用分子化学实验室

摘要(Abstract)：

为明确抗生素药物诺氟沙星对锦鲤的毒性效应,采用鱼类毒性试验方法,研究了诺氟沙星(Norfloxacin,NFLX)对锦鲤的急性毒性和亚急性毒性效应。结果表明,当NFLX暴露质量浓度达到1 000mg/L时,NFLX对锦鲤无急性毒性。NFLX暴露15 d,在1 mg/L、5mg/L、25 mg/L和125 mg/L 4个组中,5 mg/L组锦鲤肝脏SOD活性在6 d时达到最大(p<0.01);125 mg/L组MDA含量在15 d时达到最大值(p<0.01);25 mg/L、125 mg/L组锦鲤肝脏中MDA含量与暴露时间呈正相关,相关系数分别为0.938 1、0.953 5。各组GST活性和GSH质量比均受到不同程度的诱导,1 mg/L组NFLX可使鱼肝GST与GSH产生适应性反应;5 mg/L组诱导GST与GSH效应最为显著,呈现"钟形"变化趋势,5 mg/L很可能是NFLX对GST活性和GSH质量比影响的一个阈值或转折点。鱼肝GPT与GOT活性变化表现出一定的相似性,均呈现先升高后降低的变化规律,其中5 mg/L组被诱导效应最为显著(p<0.01)。5 mg/L很可能是锦鲤适应NFLX暴露和机体中生物酶发挥作用的一个转折点。比较上述抗氧化和转氨酶指标的敏感性和稳定性发现,在NFLX作用下,锦鲤肝脏中GST、GSH两个指标对NFLX的反应比SOD、MDA、GPT、GOT灵敏,变化规律稳定,更适合评价水环境中NFLX暴露的毒性效应。

关键词(KeyWords)： 环境科学技术基础学科;环境毒理学;诺氟沙星;锦鲤;毒性效应;抗氧化防御系统;转氨酶

Abstract:

Keywords:

基金项目(Foundation): 河北省自然科学基金项目(B2014208068);; 河北省药用分子化学实验室开放基金项目;; 河北省环保厅公益课题;; 河北省重点学科建设项目

作者(Authors): 沈洪艳;曹志会;赵月;王冰;

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