刘家女;房晓婷;王文静;

• 1:南开大学环境科学与工程学院
• 2:环境污染过程与基准教育部重点实验室/天津市城市生态环境修复与污染防治重点实验室

摘要(Abstract)：

根际土壤微生物是植物修复及其强化修复技术的重要组成部分,了解根际土壤微生物生态效应有助于深入探索修复机制,从而进一步提高修复效率。微生物生态效应反映土壤生态变化,对于有效评估强化调控生态风险具有重要意义。在总结污染土壤植物修复根际微生物生态效应研究的基础上,综述强化调控措施与根际土壤微生物可能存在的相互影响,并分析利用微生物分子生态学技术评估强化调控措施施用风险的可行性。

关键词(KeyWords)： 环境学;强化措施;植物修复;根际微生物;分子生物学技术

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学青年基金项目(31200395)

作者(Authors): 刘家女;房晓婷;王文静;

参考文献(References)：

• [1]WEI S,ZHOU Q,SAHA U K.Hyperaccumulative characteristics of weed species to heavy metals[J].Water,Air,and Soil Pollution,2008,192(1/2/3/4):173-181.
• [2]LI Zhen(李稹),HUANG Juan(黄娟),JIANG Lei(姜磊),et al.Research advances in the relation between plant root exudates and rhizosphere micro-environment in the made-made wetlands[J].Journal of Safety and Environment(安全与环境学报),2012,12(5):41-45.
• [3]ZHOU Qixing(周启星),SONG Yufang(宋玉芳).Technological implications of phytoremediation and its application in environment protection[J].Journal of Safety and Environment(安全与环境学报),2001,1(3):48-53.
• [4]CAI Z,ZHOU Q,PENG S,et al.Promoted biodegradation and microbiological effects of petroleum hydrocarbons by Impatiens balsamina L.with strong endurance[J].Journal of Hazardous Materials,2010,183(1):731-737.
• [5]KHAN M S,ZAIDI A,WANI P A,et al.Role of plant growth promoting rhizobacteria in the remediation of metal contaminated soils[J].En-vironmental Chemistry Letters,2009,7(1):1-19.
• [6]DO NASCIMENTO C W A,AMARASIRIWARDENA D,XING B.Comparison of natural organic acids and synthetic chelates at enhancing phytoextraction of metals from a multi-metal contaminated soil[J].Environmental Pollution,2006,140(1):114-123.
• [7]MUNN J,JANUARY M,CUTRIGHT T J.Greenhouse evaluation of EDTA effectiveness at enhancing Cd,Cr,and Ni uptake in Helianthus annuus and Thlaspi caerulescens[J].Journal of Soils and Sediments,2008,8(2):116-122.
• [8]WANG Lin(王林),ZHOU Qixing(周启星).Chemical and engineering measures applied to enhancing the phytoremediation of heavy metal contaminated soils[J].Journal of Safety and Environment(安全与环境学报),2007,7(5):50-56.
• [9]BULGARELLI D,ROTT M,SCHLAEPPI K,et al.Revealing structure and assembly cues for Arabidopsis root-inhabiting bacterial microbiota[J].Nature,2012,488(7409):91-95.
• [10]ABHILASH P C,POWELL J R,SINGH H B,et al.Plant-microbe interactions:novel applications for exploitation in multipurpose remediation technologies[J].Trends in Biotechnology,2012,30(8):416-420.
• [11]GLICK B R.Using soil bacteria to facilitate phytoremediation[J].Biotechnology Advances,2010,28(3):367-374.
• [12]MIRANSARI M.Hyperaccumulators,arbuscular mycorrhizal fungi and stress of heavy metals[J].Biotechnology Advances,2011,29(6):645-653.
• [13]YANG Q,TU S,WANG G,et al.Effectiveness of applying arsenate reducing bacteria to enhance arsenic removal from polluted soils by Pteris vittata L[J].International Journal of Phytoremediation,2012,14(1):89-99.
• [14]KUIPER I,LAGENDIJK E L,Bl OEMBERG G V,et al.Rhizoremediation:a beneficial plant-microbe interaction[J].Molecular Plant-Microbe Interactions,2004,17(1):6-15.
• [15]BRAUD A,JEZEQUEL K,BAZOT S,et al.Enhanced phytoextraction of an agricultural Cr-and Pb-contaminated soil by bioaugmentation with siderophore-producing bacteria[J].Chemosphere,2009,74(2):280-286.
• [16]DELVASTO P,BALLLESTER A,MUNOZ J A,et al.Mobilization of phosphorus from iron ore by the bacterium Burkholderia caribensis Fe GL03[J].Minerals Engineering,2009,22(1):1-9.
• [17]HAN F,SHAN X,ZHANG S,et al.Enhanced cadmium accumulation in maize roots—the impact of organic acids[J].Plant and Soil,2006,289(1/2):355-368.
• [18]VENKATESH N M,VEDARAMAN N.Remediation of soil contaminated with copper using rhamnolipids produced from Pseudomonas aeruginosa MTCC 2297 using waste frying rice bran oil[J].Annals of Microbiology,2012,62(1):85-91.
• [19]JOSHI P M,JUWARKAR A A.In vivo studies to elucidate the role of extracellular polymeric substances from Azotobacter in immobilization of heavy metals[J].Environmental Science&Technology,2009,43(15):5884-5889.
• [20]SHI J Y,LIN H R,YUAN X F,et al.Enhancement of copper availability and microbial community changes in rice rhizospheres affected by sulfur[J].Molecules,2011,16(2):1409-1417.
• [21]KRUPA P,KOZDROJ J.Ectomycorrhizal fungi and associated bacteria provide protection against heavy metals in inoculated pine(Pinus sylvestris L.)seedlings[J].Water,Air,and Soil Pollution,2007,182(1/2/3/4):83-90.
• [22]DAKORA F D,PHILLIPS D A.Root exudates as mediators of mineral acquisition in low-nutrient environments[J].Plant and Soil,2002,245(1):35-47.
• [23]BAIS H P,WEIR T L,PERRY L G,et al.The role of root exudates in rhizosphere interactions with plants and other organisms[J].Annual Review of Plant Biology,2006,57:233-266.
• [24]WEYENS N,VAN DER LELIE D,TAGHAVI S,et al.Exploiting plant-microbe partnerships to improve biomass production and remediation[J].Trends in Biotechnology,2009,27(10):591-598.
• [25]PARIDA B K,CHHIBBA I M,NAYYAR V K.Influence of nickel-contaminated soils on fenugreek(Trigonella corniculata L.)growth and mineral composition[J].Scientia Horticulturae,2003,98(2):113-119.
• [26]OUZOUNIDOU G,MOUSTAKAS M,SYMEONIDIS L,et al.Response of wheat seedlings to Ni stress:effects of supplemental calcium[J].Archives of Environmental Contamination and Toxicology,2006,50(3):346-352.
• [27]SARAVANAN V S,MADHAIYAN M,THANGARAJU M.Solubilization of zinc compounds by the diazotrophic,plant growth promoting bacterium Gluconacetobacter diazotrophicus[J].Chemosphere,2007,66(9):1794-1798.
• [28]SALEH S,HUANG X D,GREENBERG B M,et al.Phytoremediation of persistent organic contaminants in the environment[M]//SIGH A,WARD O P.Applied bioremediation and phytoremediation.Berlin,Heidelberg:Springer,2004:115-134.
• [29]ZHUANG X,CHEN J,SHIM H,et al.New advances in plant growth-promoting rhizobacteria for bioremediation[J].Environment International,2007,33(3):406-413.
• [30]HUANG X D,EL-ALAWI Y,GURSKA J,et al.A multi-process phytoremediation system for decontamination of persistent total petroleum hydrocarbons(TPHs)from soils[J].Microchemical Journal,2005,81(1):139-147.
• [31]MURATOVAA Y,TURKOVSKAYA O V,ANTONYUK L P,et al.Oil-oxidizing potential of associative rhizobacteria of the genus Azospirillum[J].Microbiology,2005,74(2):210-215.
• [32]CHAUDHRY Q,BLOM-ZANDSTRA M,GUPTA S K,et al.Utilizing the synergy between plants and rhizosphere microorganisms to enhance breakdown of organic pollutants in the environment(15pp)[J].Environmental Science and Pollution Research,2005,12(1):34-48.
• [33]MACKOVA M,PROUZOVA P,STURSA P,et al.Phyto/rhizoremediation studies using long-term PCB-contaminated soil[J].Environmental Science and Pollution Research,2009,16(7):817-829.
• [34]DANDIE C E,THOMAS S M,BENTHAM R H,et al.Physiological characterization of Mycobacterium sp.strain 1B isolated from a bacterial culture able to degrade high-molecular-weight polycyclic aromatic hydrocarbons[J].Journal of Applied Microbiology,2004,97(2):246-255.
• [35]ZHANG X,WANG Z,LIU X,et al.Degradation of diesel pollutants in Huangpu-Yangtze River estuary wetland using plant-microbe systems[J].International Biodeterioration&Biodegradation,2013,76:71-75.
• [36]ZHOU Qixing(周启星),CHENG Yun(程云),ZHANG Qianru(张倩茹),et al.Quantitative analysis of combination contamination ecotoxicological effects[J].Science in China:Series C(中国科学:C辑),2003,33(6):566-573.
• [37]CHATTERJEE S,SAU G B,MUKHERJEE S K.Plant growth promotion by a hexavalent chromium reducing bacterial strain,Cellulosimicrobium cellulans KUCr3[J].World Journal of Microbiology and Biotechnology,2009,25(10):1829-1836.
• [38]ZHU Zhiqiang(朱志强).Plant-microbe remediation of Cd-DDT co-contaminated soils and its mechanisms(Cd-DDT复合污染土壤的植物与微生物联合修复及机理)[D].Hangzhou:Zhejiang University,2012.
• [39]RUPPERT L,LIN Z Q,DIXON R P,et al.Assessment of solid phase microfiber extraction fibers for the monitoring of volatile organoarsinicals emitted from a plant-soil system[J].Journal of Hazardous Materials,2013,262:1230-1236.
• [40]ZHOU J,LI X,JIANG Y,et al.Combined effects of bacterial-feeding nematodes and prometryne on the soil microbial activity[J].Journal of Hazardous Materials,2011,192(3):1243-1249.
• [41]ZHANG H,DANG Z,ZHENG L C,et al.Remediation of soil co-contaminated with pyrene and cadmium by growing maize(Zea mays L.)[J].International Journal of Environmental Science&Technology,2009,6(2):249-258.
• [42]MENCH M,SCHWITZGUEBEL J P,SCHROEDER P,et al.Assessment of successful experiments and limitations of phytotechnologies:contaminant uptake,detoxification and sequestration,and consequences for food safety[J].Environmental Science and Pollution Research,2009,16(7):876-900.
• [43]PERRY V R,KROGSTAD E J,El-MAYAS H,et al.Chemically enhanced phytoextraction of lead-contaminated soils[J].International Journal of Phytoremediation,2012,14(7):703-713.
• [44]ULTRA JR V U,YANO A,IWASAKI K,et al.Influence of chelating agent addition on copper distribution and microbial activity in soil and copper uptake by brown mustard(Brassica juncea)[J].Soil Science&Plant Nutrition,2005,51(2):193-202.
• [45]XU Jun(徐军).Study on the effects and mechanism of promoting rhizobacteria and EDTA on plant growth and accumulation of heavy metals in the soil(植物促生细菌和EDTA对植物生长与富集土壤重金属的影响及机制研究)[D].Nanjing:Nanjing Agricultural University,2012.
• [46]ZOU Xing(邹星).The research of chemical strengthening Sedum alfredii H's remediation on the composite pollution of heavy metals-BDE-209(化学强化东南景天修复土壤重金属-十溴联苯醚复合污染的研究)[D].Jinan:Jinan University,2011.
• [47]SHAHID M,PINELLI E,DUMAT C.Review of Pb availability and toxicity to plants in relation with metal speciation,role of synthetic and natural organic ligands[J].Journal of Hazardous Materials,2012,219:1-12.
• [48]LOMB E,ZHAO F J,DUNHAM S J,et al.Phytoremediation of heavy metal-contaminated soils[J].Journal of Environmental Quality,2001,30(6):1919-1926.
• [49]ZHAO S,SHANG X,DUO L.Accumulation and spatial distribution of Cd,Cr,and Pb in mulberry from municipal solid waste compost following application of EDTA and(NH4)2SO4[J].Environmental Science and Pollution Research,2013,20(2):967-975.
• [50]MUHLBACHOVA’G.Soil microbial activities and heavy metal mobility in long-term contaminated soils after addition of EDTA and EDDS[J].Ecological Engineering,2011,37(7):1064-1071.
• [51]MATTINAM J I,IANNUCCI-BERGER W,EITZER B D,et al.Rhizotron study of Cucurbitaceae:transport of soil-bound chlordane and heavy metal contaminants differs with genera[J].Environmental Chemistry,2004,1(2):86-89.
• [52]GLICK B R.Phytoremediation:synergistic use of plants and bacteria to clean up the environment[J].Biotechnology Advances,2003,21:383-393.
• [53]LYNCH J M,MOFFAT A J.Bioremediation-prospects for the future application of innovative applied biological research[J].Annals of Applied Biology,2005,146(2):217-221.
• [54]HASTINGS R C,SAUNDER J R,HALL G H,et al.Application of molecular biological techniques to a seasonal study of ammonia oxidation in a eutrophic freshwater lake[J].Applied and Environmental Microbiology,1998,64(10):3674-3682.
• [55]YANG Yonghua(杨永华),YAO Jian(姚健).Molecular techniques and their application to the study of microbial diversity[J].Chinese Biodiversity(生物多样性),2000,8(3):337-342.
• [56]MUYZER G,SMALLA K.Application of denaturing gradient gel electrophoresis(DGGE)and temperature denaturing gradient gel electrophoresis(TGGE)in microbial ecology[J].Antonie Van Leeuwenhoek,1998,73(1):127-135.
• [57]MUYZER G.DGGE/TGGE a method for identifying genes from natural eco systems[J].Current Microbiology,1999,2:317-322.
• [58]ROY S,LABELLE S,MEHTA P,et al.Phytoremediation of heavy metal and PAH-contaminated brownfield sites[J].Plant and Soil,2005,272(1/2):277-290.
• [59]SCHAFER H,BERNARD L,COURTIES C,et al.Microbial community dynamics in Mediterranean nutrient-enriched seawater mesocosms:changes in the genetic diversity of bacterial populations[J].FEMS Microbiology Ecology,2001,34(3):243-253.
• [60]KIRK J L,KLIRONOMOS J N,LEE H,et al.The effects of perennial ryegrass and alfalfa on microbial abundance and diversity in petroleum contaminated soil[J].Environmental Pollution,2005,133(3):455-465.
• [61]SICILIANO S D,GERMIDA J J,BANKS K,et al.Changes in microbial community composition and function during a polyaromatic hydrocarbon phytoremediation field trial[J].Applied and Environmental Microbiology,2003,69(1):483-489.
• [62]CHEN Y X,WANG Y P,WU W X,et al.Impacts of chelate-assisted phytoremediation on microbial community composition in the rhizosphere of a copper accumulator and non-accumulator[J].Science of the Total Environment,2006,356(1):247-255.
• [63]PALMROTH M R T,KOSKINEN P E P,KAKSONEN A H,et al.Metabolic and phylogenetic analysis of microbial communities during phytoremediation of soil contaminated with weathered hydrocarbons and heavy metals[J].Biodegradation,2007,18(6):769-782.
• [64]ZHAO Jia(赵佳),SUN Yi(孙毅),LIANG Hong(梁宏),et al.The application of modern biotechnology in the research of rhizosphere microbial community[J].Biotechnology Bulletin(生物技术通报),2012(12):65-70.
• [65]FODOR S P,READ J L,PIRRUNG M C,et al.Light-directed,spatially addressable parallel chemical synthesis[J].Science,1991,251:767-773.
• [66]HUO Jinlong(霍金龙),MIAO Yongwang(苗永旺),ZENG Yangzhi(曾养志).Gene chip technology and its application[J].Letters in Biotechnology(生物技术通讯),2007,18(2):329-331.
• [67]RAO M R,HALFHILL M D,ABERCROMBIE L G,et al.Phytoremediation and phytosensing of chemical contaminants,RDX and TNT:identification of the required target genes[J].Functional&Integrative Genomics,2009,9(4):537-547.
• [68]PENG R H,XU R R,FU X Y,et al.Microarray analysis of the phytoremediation and phytosensing of occupational toxicant naphthalene[J].Journal of Hazardous Materials,2011,189(1):19-26.
• [69]GAO J J,SHEN X F,SHENG R H,et al.Phytoremediation and phytosensing of chemical contaminant,toluene:identification of the required target genes[J].Molecular Biology Reports,2012,39(8):8159-8167.
• [70]ZHONG Yi(钟毅),ZHANG Xu(张旭),LIANG Yuting(梁玉婷),et al.Microbial community structure in oil contaminated soil using the Geo Chip technique[J].Journal of Tsinghua University:Science&Technology(清华大学学报:自然科学版),2010,50(9):1396-1399.
• [71]SANGUIN H,REMENANT B,DECHESNE A,et al.Potential of a16S rRNA-based taxonomic microarray for analyzing the rhizosphere effects of maize on Agrobacterium spp.and bacterial communities[J].Applied and Environmental Microbiology,2006,72(6):4302-4312.
• [72]LIN Zhong(蔺中),SUN Liyong(孙礼勇),CHEN Hao(陈昊),et al.Application of stable isotope probing on in-situ identification of soil functional microorganism[J].Journal of Agro-Environment Science(农业环境科学学报),2012,31(1):1-6.
• [73]BOSCHKER H T S,NOLD S C,WELLSBURY P,et al.Direct linking of microbial populations to specific biogeochemical processes by13C-labelling of biomarkers[J].Nature,1998,392(6678):801-805.
• [74]FRIENCH M W.Stable-isotope probing of DNA:insights into the function of uncultivated micro-organisms from isotopically labeled metagenomes[J].Current Opinion in Biotechnology,2006,17(1):59-66.
• [75]SCHWARTZ E.Characterization of growing microorganisms in soil by stable isotope probing with(H2O)-O-18[J].Applied and Environmental Microbiology,2007,73(8):2541-2546.
• [76]XIA W,ZHANG C,ZENG X,et al.Autotrophic growth of nitrifying community in an agricultural soil[J].The ISME Journal,2011,5(7):1226-1236.
• [77]MAIYSHA D J,DOUGLAS W C,DAVID R S,et al.Stable-isotope probing of the polycyclic aromatic hydrocarbon-degrading bacterial guild in a contaminated soil[J].Environmental Microbiology,2011,13(10):2623-2632.
• [78]PROUZOVáP,HOSKOVCOVA E,MUSILOVA L,et al.The effect of plants and natural compounds on bacterial population in the contaminated soil[C]//Proc of the 5th International Symposium on Biosorption and Bioremediation(Bio Bio).Prague:ICT Press,2012:60-63.
• [79]HAICHAR FZ,ACHOUAK W,CHRISTEN R,et al.Identification of cellulolytic bacteria in soil by stable isotope probing[J].Environmental Microbiology,2007,9(3):625-634.
• [80]LU Y,LUEDERS T,FRIEDRICHM W,et al.Detecting active methanogenic populations on rice roots using stable isotope probing[J].Environmental Microbiology,2005,7(3):326-336.