谢宇轩,关翔宇,王阳,王鹤立

• 1:中国地质大学(北京)水资源与环境学院
• 2:中国地质大学(北京)海洋学院

摘要(Abstract)：

了解特定环境中高氯酸盐(ClO-4)降解菌的群落组成,对ClO-4的降解具有重要的指导意义。通过添加醋酸盐作为电子供体降解ClO-4,利用高通量测序(HiSeq 2000)的方法获得了复杂环境中生物群落的组成,对比了降解前后菌种结构的变化。结果表明,补充醋酸盐的降解体系(Acetate Degradation,AD)将10 mg/L ClO-4降至检出限以下需100 h。降解完毕后代表性的ClO-4降解菌Dechloromonas的相对丰度为0.2%,与原始活性污泥相比无明显差别;另一典型ClO-4降解菌脱氯菌属Azospira相对丰度为3.2%;一些同时参与ClO-4降解和脱氮作用的细菌如假单胞菌属Pseudomonas也有检出,相对丰度为8%。异养条件下ClO-4降解是假单胞菌属Pseudomonas和脱氯菌属Azospira起主导作用。AD体系内菌种多样性小于原始活性污泥。电子供体的加入使活性污泥类的混合体系内生物群落结构单一化,使降解基质具备了在特定环境下针对某种污染物降解的能力。

关键词(KeyWords)： 环境工程学;高氯酸盐;生物降解;醋酸盐;高通量测序;细菌群落变化

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(41272268);; 中央高校基本科研业务费专项资金项目(2652012071);; 中国地质调查局项目(1212011121171)

作者(Author): 谢宇轩,关翔宇,王阳,王鹤立

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