pH值对白茅生物质光合生物产氢的影响Effect of pH values on cogon photosynthetic hydrogen production
荆艳艳;周小楷;李芳;王震宁;张全国;
摘要(Abstract):
为了探究pH值对白茅光合产氢体系产氢效果的影响,使用白茅生物质作为产氢原料,以产氢速率和产氢量为关键指标、以还原糖质量浓度和氧化还原电位(ORP)等为辅助指标研究pH值对白茅光合生物产氢的影响,分析几种指标的数据变化情况,提出pH值对白茅光合生物产氢的影响规律。结果表明,ORP呈现先降低后上升的趋势。当pH=8时,光合细菌对白茅降解的还原糖利用率较高,超过了94.8%;累计产氢量和产氢速率达到最大,分别为422 mL和8.08 mL/h, ORP达到最小值-465 mV。试验结束时各体系的pH值维持在5.3~5.5,中性和碱性环境下白茅光合生物产氢能力明显高于酸性环境下的产氢能力,但碱性过强又对产氢能力产生抑制作用。
关键词(KeyWords): 环境工程学;pH值;光合生物制氢;白茅;生物质
基金项目(Foundation): 国家自然科学基金项目(52076068);; 河南省高校科技创新团队支持计划项目(20IRTSTHN006)
作者(Authors): 荆艳艳;周小楷;李芳;王震宁;张全国;
DOI: 10.13637/j.issn.1009-6094.2020.1792
参考文献(References):
- [1] 宋金龙.燃料使用对环境的影响[J].中国环境管理丛书,2008(3):110.SONG J L.Impact of fuel use on environment[J].China Environmental Management Series,2008(3):110.
- [2] 王承平.新能源和可再生能源的开发与利用讨论[J].南方农机,2019,50(1):238.WANG C P.Discussion on the development and utilization of new energy and renewable energy[J].Southern Agricultural Machinery,2019,50(1):238.
- [3] 申翔伟,周雪花,杜金宇,等.生物制氢技术发展历程及其特征[J].太阳能,2010(1):22-25.SHEN X W,ZHOU X H,DU J Y,et al.Development history and characteristics of biohydrogen production technology[J].Solar Energy,2010(1):22-25.
- [4] LU C,ZHANG H,ZHANG Q,et al.An automated control system for pilot-scale biohydrogen production:design,operation and validation[J].International Journal of Hydrogen Energy,2020,45(6):3795-3806.
- [5] ZHU G,WU T,JHAA K,et al.Review of bio-hydrogen production and new application in the pollution control via microbial electrolysis cell[J].Desalination and Water Treatment,2014,52(28/29/30):5413-5421.
- [6] 隋琼.秸秆类生物质光合制氢发酵尾液的浸种和育苗实验研究[D].郑州:河南农业大学,2019.SUI Q.Experimental study on soaking seed and seedling of straw biomass fermentation tailliquor for hydrogen production by photosynthesis[D].Zhengzhou:Henan Agricultural University,2019.
- [7] 徐洋,蔺占兵,马庆虎.木质素与人类生活[J].植物杂志,2002(4):40-41.XU Y,LIN Z B,MA Q H.Lignin and human life[J].Botanic Journal,2002(4):40-41.
- [8] 邓艳辉.白茅菅草的再生综合利用基础研究[D].长春:吉林大学,2009.DENG Y H.Basic research on regeneration and comprehensive utilization of Sedge of Cogongrass and Kan grass[D].Changchun:Jilin University,2009.
- [9] 范树国,周丽娟,邱璐,等.硫酸法测定4种常见农作物秸秆的木质素含量[J].江苏农业科学,2010,38(5):395-398.FAN S G,ZHOU L J,QIU L,et al.Determination of lignin content in 4 common crop straws by sulfuric acid method[J].Jiangsu Agricultural Sciences,2010,38(5):395-398.
- [10] 路朝阳,王毅,曹明,等.pH值对玉米秸秆酶解液光合生物产氢动力学的影响[J].安全与环境学报,2016,16(3):262-266.LU C Y,WANG Y,CAO M,et al.Effects of pH on hydrodynamics of photosynthetic organisms in enzymatic hydrolysate of corn stalks[J].Chinese Journal of Safety and Environment,2016,16(3):262-266.
- [11] NATH K,DAS D.Effect of light intensity and initial pH during hydrogen production by an integrated dark and photofermentation process[J].International Journal of Hydrogen Energy,2009,34(17):7497-7501.
- [12] 蒋丹萍,韩滨旭,王毅,等.HAU-M1光合产氢细菌的生理特征和产氢特性分析[J].太阳能学报,2015,36(2):289-294.JIANG D P,HAN B X,WANG Y,et al.Physiological characteristics and hydrogen-producing characteristics of HAU-M1 photosynthetic hydrogen-producing bacteria[J].Journal of Solar Energy,2015,36(2):289-294.
- [13] 张甜.产气肠杆菌AS1.489与光合细菌HAU-M1共发酵生物制氢研究[D].郑州:河南农业大学,2018.ZHANG T.Study on hydrogen production by co-fermentation of enterobacteriaceae AS1.489 and photosynthetic bacterium HAU-M1[D].Zhengzhou:Henan Agricultural University,2018.
- [14] 张全国,刘会亮,胡建军,等.磷酸盐和碳酸盐对秸秆类生物质光发酵产氢的影响[J].农业工程学报,2017,33(13):251-257.ZHANG Q G,LIU H L,HU J J,et al.Effects of phosphate and carbonate on hydrogen production by light fermentation of straw biomass[J].Journal of Agricultural Engineering,2017,33(13):251-257.
- [15] 岳建芝,李刚,焦有宙,等.光照度对酶解秸秆料液光合产氢的影响[J].农业工程学报,2011,27(8):313-317.YUE J Z,LI G,JIAO Y Z,et al.Effect of light intensity on hydrogen production from enzymatic hydrolysis straw feed solution[J].Journal of Agricultural Engineering,2011,27(8):313-317.
- [16] 朱胜楠.光合生物制氢过程中扰动效应的实验研究[D].郑州:河南农业大学,2019.ZHU S N.Experimental study on disturbance effect during hydrogen production by photosynthetic organisms[D].Zhengzhou:Henan Agricultural University,2019.
- [17] 方心芳,刘书田,刘肃,等.金霉菌发酵液的氧化还原电位与金霉素效价变化的试验[J].科学通报,1957,2(23):720.FANG X F,LIU S T,LIU S,et al.Experimental study on REDOX potential and aureomycin titer change of aureomycin fermentation broth[J].Chinese Science Bulletin,1957,2(23):720.
- [18] OKTYABRSKII O N,SMIRNOVA G V.Redox potential changes in bacterial cultures under stress conditions[J].Microbiology,2012,81(2):131-142.