铀胁迫对香根草生理生化指标的影响Effects of uranium stress on the physiological and biochemical indexes of Vetiveria zizanioides
赵聪;谢水波;李仕友;魏庆鹏;
摘要(Abstract):
采用水培方式研究了不同质量浓度(0、0.1 mg/L、1 mg/L、5mg/L、10 mg/L、20 mg/L)铀胁迫下香根草光合色素质量比、可溶性蛋白质质量比、丙二醛(MDA)含量、抗氧化酶(超氧化物歧化酶SOD、过氧化物酶POD、过氧化氢酶CAT)活性、非酶物质(非蛋白巯基NPT、谷胱甘肽GSH和植物络合素PCs)含量的变化。结果表明,铀质量浓度为0.1 mg/L和1 mg/L时,可促进香根草光合色素和可溶性蛋白质质量比的增加,但随铀质量浓度增加,光合色素和可溶性蛋白质质量比逐渐下降。铀胁迫诱导香根草体内MDA含量、POD活性和CAT活性呈明显升高的趋势,SOD活性则表现为逐渐下降的趋势。不同质量浓度铀胁迫对香根草根部NPT、GSH和PCs含量无明显影响,铀质量浓度为0.1 mg/L时香根草根部NPT、GSH和PCs含量略有增加;随铀胁迫质量浓度增加,叶片内GSH含量逐渐下降,NPT和PCs含量则逐渐升高。
关键词(KeyWords): 环境工程学;香根草;铀浓度;抗氧化酶;植物络合素
基金项目(Foundation): 国家自然科学基金项目(11175081);; 湖南省科技计划重点项目(2011SK2015);; 湖南省高校创新平台开放基金项目(13K085,13K086)
作者(Authors): 赵聪;谢水波;李仕友;魏庆鹏;
参考文献(References):
- [1]NEVES O,ABREU M M,VICENTE E M.Uptake of uranium by lettuce(Lactuca sativa L.)in natural uranium contaminated soils in order to assess chemical risk for consumers[J].Water,Air,and Soil Pollution,2008,195(1/2/3/4):73-84.
- [2]MIRJANA D S,DRAGI R S,JELENA V M,et al.Phytotoxic effect of the uranium on the growing up and development the plant of corn[J].Water,Air,and Soil Pollution,2010,209(1/2/3/4):401-410.
- [3]ZHANG Xueli(张学礼),WANG Erqi(王尔奇).Review on phytoremediation of uranium-contaminated environment[J].Uranium Mining and Metallurgy(铀矿冶),2008,27(1):44-49.
- [4]SHIN D C,KIM Y S,MOON J Y,et al.International trends in risk management of groundwater radionuclides[J].Journal of Environmental Toxicology,2002,17(4):273-284.
- [5]XIE S B,YANG J,CHEN C,et al.Study on biosorption kinetics and thermodynamics of uranium by Citrobacter freudii[J].Journal of Environmental Radioactivity,2008,99(1):126-133.
- [6]WAN Qinfang(万芹芳),REN Yamin(任亚敏),WANG Liang(王亮),et al.Phytoremediation for soil contaminated by uranium[J].Acta Chimica Sinica(化学学报),2011,69(15):1780-1788.
- [7]SUN Lei(孙磊).Study on phytoremediation of heavy metal pollution in soil(土壤重金属污染的植物修复研究)[D].Changchun:Jilin University,2009.
- [8]XIA Hanping(夏汉平),AO Huixiu(敖惠修),HE Daoquan(何道全),et al.A study on vetiver grass in soil amelioration and soil and moisture conservation[J].Tropical Geography(热带地理),1996,16(3):265-270.
- [9]LIN Jie(林杰),KE Jinlian(柯金炼),WEI Daojun(魏道军).Effect of lead stress on membrane and antioxidant systems in Vetiveria zizanioides leaves[J].Fujian Journal of Agricultural Sciences(福建农业学报),2009,24(3):254-257.
- [10]NUZA’AITI·Aibibu(努扎艾提·艾比布),LIU Yunguo(刘云国),ZENG Guangming(曾光明),et al.Cadmium tolerance and accumulation characteristics of Vetiveria zizanioides[J].Acta Scientiae Circumstantiae(环境科学学报),2009,29(9):1958-1963.
- [11]XIONG Jun(熊俊),YUAN Xi(袁喜),WANG Fei(王飞),et al.The effects of Pb pollution on physiological and biochemical characters of vetiver grass and Liriope[J].Environment and Ecology in the Three Gorges(三峡环境与生态),2010,32(6):9-12.
- [12]YAN Zheng(严政),XIE Shuibo(谢水波),LI Shiyou(李仕友),et al.Physiological and biochemical responses of Eichhornia crassipes and Pistia stratiotes induced at uranium stress[J].Journal of Safety and Environment(安全与环境学报),2012,12(3):1-5.
- [13]LI Hesheng(李合生).Principles and techniques of plant physiological biochemical experiment(植物生理生化实验原理和技术)[M].Beijing:Higher Education Press,2000:134-137.
- [14]WANG Xuekui(王学奎).Principles and techniques of plant physiological biochemical experiment(植物生理生化实验原理和技术)[M].2nd ed.Beijing:Higher Education Press,2006:167-173.
- [15]TIJEN D,ISMAIL T.Comparative lipid peroxidation,antioxidant defense systems and proline content in roots of two rice cultivars differing in salt tolerance[J].Environmental and Experimental Botany,2005,53(2):247-257.
- [16]ELLMAN G L.Tissue sulphydryl groups[J].Archives of Biochemistry and Biophysics,1959,82(1):70-77.
- [17]CAO X,MA Q L,TU C.Antioxidative responses to arsenic in the arsenic-hyperaccumulator Chinese brake fern(Pteris vittata L.)[J].Environmental Pollution,2004,128(3):317-325.
- [18]SHEPPARD S C,SHEPPARD M I,GALLERAND M O,et al.Derivation of ecotoxicity thresholds for uranium[J].Journal of Environmental Radioactivity,2005,79(1):55-83.
- [19]JENNIFER C,NORA G.Cleaning up contaminants with plants[J].Biocycle,2005,46(8):30-32.
- [20]NUZA’AITI·Aibibu(努扎艾提·艾比布),LIU Yunguo(刘云国),SONG Huaxiao(宋华晓),et al.Effects of Zn and Cu on physiological and biochemical processes and their accumulation characteristics of vetiver[J].Journal of Agro-Environment Science(农业环境科学学报),2010,29(1):54-59.
- [21]ZHANG Kai(张凯).Study on tolerance mechanism of hypertolerant plant Conyza canadensis to cadmium(镉富集植物小飞蓬对镉的耐性机制研究)[D].Fuzhou:Fujian Agriculture and Forestry University,2010.
- [22]SRIVASTAVA S,BHAINSA K C,D’SOUZA S F.Investigation of uranium accumulation potential and biochemical responses of an aquatic weed Hydrilla verticillata(L.f.)Royle[J].Bioresource Technology,2010,101(8):2573-2579.
- [23]PENG L,PARDEE A B.Differential display of eukaryotic messenger RNA by means of the polymerase chain reaction[J].Science,1992,257(5072):967-971.
- [24]ZHANG Zhongchun(张中春),QIU Baosheng(邱保胜).Research advances of phytochelatin transport and its function[J].Plant Physiology Journal(植物生理学报),2012,48(5):425-428.
- [25]GRILL E,WINNACKER E-L,ZEN K M H.Phytochelatins,a class of heavy-metal-binding peptides from plants,are functionally analogous to metallothioneins[J].Proceedings of the National Academy of Sciences of the United States of America,1987,84(2):439-443.
- [26]YU Fangming(于方明),TANG Yetao(汤叶涛),QIU Rongliang(仇荣亮),et al.Antioxidative responses to cadmium stress in the hyperaccumulator Arabis paniculata Franch[J].Acta Scientiae Circumstantiae(环境科学学报),2010,30(2):409-414.